Ontario Public Health says 28 of the 33 cases are in Toronto and all confirmed cases so far have been in men.
“In addition, there are 11 possible cases and 34 suspicious cases.  “These 45 possible and suspected cases include 11 women and 34 men aged <20 to 71 years (average: 34.3) and were reported by nine public health units," Public Health Ontario wrote in a document released Tuesday. .
Middlesex-London, Ottawa, Halton and Wellington-Dufferin-Guelph also have confirmed cases.
Suspicious cases have been reported in Hamilton, Durham, Eastern Ontario, York and Windsor-Essex.
Earlier this month, Toronto Public Health said it was aware of 18 confirmed cases of smallpox in monkeys.
Across Canada, there are 168 confirmed cases, 141 of them in Quebec.
The World Health Organization said last week that it knows of 2,103 confirmed laboratory cases worldwide, including one death.
In all confirmed cases, the most common symptoms reported are skin rash, oral or genital lesions, swollen lymph nodes, fever or chills.
While anyone can catch and transmit monkey pox, the recent outbreak in Europe and North America has seen a significant spread among homosexuals, bisexuals and men having sex with men.
In response, Toronto Public Health is giving the Imvamune smallpox vaccine to bath workers and patrons, sex workers and men who have sex with men who have had two or more sexual partners in the past three weeks.
Cisgender women are not eligible for the Imvamune vaccine unless they are identified as having close contact with a previously confirmed case.
Federal guidelines indicate that the smallpox vaccine is most effective if given within four days of exposure to a case, but may help if given up to 14 days after exposure.
Monkey pox is usually transmitted through prolonged close contact between people who are breathing, talking, coughing or sneezing.
It can also spread through skin-to-skin contact with rashes or body fluids and can also remain on objects such as clothing or sheets that have come in contact with an infected person.
Symptoms can occur anywhere between 5 and 21 days after exposure.
Vaccination clinics operate this week at Metro Hall, Cloverdale Mall in Etobicoke and 1940 Eglinton Avenue East in Scarborough.